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Semen should be examined before use or purchased from a reputable source and properly handled. This also supports consistency in inseminator skill and prevents inseminator fatigue. The treatment protocol that utilizes GnRH and prostaglandin for fixed-time AI in dairy cattle was first referred to as Ovsynch. Genetic evaluation of beef bulls has improved considerably in recent years, making bull selection more objective and reliable. The importance of body condition, postpartum interval, pubertal status and nutrition cannot be overemphasized. However, it is underutilized in the U.S. beef herds. Although MGA is marketed to suppress estrus in feedlot heifers, it has been used in various regimens to synchronize estrus. If heifers are cyclng the first GnRH can be omitted and a single administration of prostaglandin is all that is required to regress the original CL. Theriogenology 77:1624-1631, 2012. Bulls of high genetic merit are available with AI. There are now two progesterone-releasing devices licensed for use for estrus synchronization in Canada, the  progesterone releasing intravaginal device (PRID)  and the controlled internal drug release (CIDR). Do not place the rod in your mouth or carry it uncovered in your hand. Bridges GA, Helser LA, Grum DE, Mussard ML, Gasser CL, Day ML: Decreasing the interval between GnRH and PGF2α from 7 to 5 days and lengthening proestrus increases timed-AI pregnancy rates in beef cows. Theriogenology 76:578-588, 2011. Environmental Footprint of Beef Production, Introduction to Record Keeping and Benchmarking, Canadian Beef Research and Technology Transfer Strategy, Award for Outstanding Research & Innovation, 2) Controlling follicular development and ovulation, Treatment Protocols for the Synchronization of Estrus and Ovulation,,,,,,,, Production Practices on Cow-Calf Operations. Immediately after the unit of semen is immersed in water, return the cane to the canister by raising the canister up over the cane. Martinez MF, Kastelic JP, Adams GP, Mapletoft RJ: The use of a progesterone-releasing device (CIDR-B) or melengestrol acetate with GnRH, LH, or estradiol benzoate for fixed-time AI in beef heifers. In this study, the number of bulls was decreased, from eight to four. Thus, the timing of AI must be adjusted according to when the final dose of either GnRH or estradiol is administered. The cause of the increased pregnancy rates was proposed to be due to a lengthened developmental period for the ovulatory follicle (proestrus) and higher estradiol levels at the time of AI. Baruselli Pietro Sampaio, Ferreira Roberta Machado, Colli Marcos Henrique Alcantara, Elliff Flávia Morag, Sá Filho Manoel Francisco, Vieira Lais, Gonzales de Freitas Bruno: Timed artificial insemination: current challenges and recent advances in reproductive efficiency in beef and dairy herds in Brazil. Use of a 6-day J-Synch protocol has resulted in higher pregnancy rates in heifers than the conventional 7- or 8-day estradiol-based protocol. Keep the canister tops no higher than the frost line, or keep the tops of the canes no higher than two to three inches from the tank’s top. Click the image to download the Excel file shown above and calculate the cost of a bull per naturally sired calf. Virginia Polytechnic Institute, Blacksburg, VA. As an alternative, it is possible to greatly reduce the number of bulls needed, especially in large herds. Wrap the assembled insemination rod in a clean, dry paper towel, and tuck it within your clothing for transportation to the cow. One method is to give two doses of prostaglandin 11-14 days apart with the idea that all animals should have a corpus luteum in the desired 6 to 17 day window at the time of the second injection. Anim Reprod Sci 86:37-52, 2005. Anim Reprod Sci 57:23-33, 1999. Estradiol is administered at the time of insertion of a progesterone device to synchronize follicle growth. Bó GA, Adams GP, Pierson RA, Mapletoft RJ: Exogenous Control of Follicular Wave Emergence in Cattle. The predominant natural estrogen in non-pregnant, reproductively competent females is estradiol-17β with the principal site of production being the follicle. Gently shake the straw as it is taken from the tank to remove any liquid nitrogen that may be retained in the cotton plug end of the straw. A maximum of eight students per instructor. However, recent studies have shown that the first GnRH results in ovulation in less than 60% of cattle. A distinct advantage to using MGA is that it is inexpensive when compared to progesterone-releasing devices. Those with three waves will have a 21 to 24-day cycle. Artificial insemination (AI) is one of the most effective tools available to cattle producers to improve productivity and profitability of their cattle operation. If the cow is non-pregnant, prostaglandin is released from the uterus late in the cycle, causing destruction of the corpus luteum; progesterone levels decrease, estrogen production from a growing follicle increases and the cow goes back into heat. After the semen is thawed for the required time, dry it thoroughly with a paper towel and protect it from rapid cooling. Martinez MF, Adams GP, Bergfelt D, Kastelic JP, Mapletoft RJ: Effect of LH or GnRH on the dominant follicle of the first follicular wave in heifers. When used alone, MGA must be fed for approximately 14 days to synchronize estrus effectively; however, follicles developing in such an environment ovulate poor quality eggs. Although artificial insemination (AI) of cattle has been possible for 60 years, this technology has not been used widely in the Canadian beef industry. If the herd is cycling normally, and estrus detection is done well, all animals in the herd should have been inseminated during this 10 day period of time. This is an excellent reference source put together by representatives from the AI and pharmaceutical industries, veterinarians, and reproductive physiologists from the Beef Reproduction Task Force. Estrogen products that have undergone a modification of their chemical structure to lengthen there duration of effect are also available e.g., estradiol benzoate and estradiol cypionate. Crocci and C. M. Barros'' Departments of 'Pharmacology and 2Statistics, Instituto de Biociencias - UNESP 18618.000 Botucatu, Silo Paulo, Brazil Received for publication: November 24, 1999 Accepted: October 18, 2000 ABSTRACT The … An estradiol-based protocol for fixed-time AI is illustrated in Figure 1. Good planning and communication, including written protocols and frequent consultations with your veterinarian, are essential. Thanks to Colin Palmer, Ph.D. and Reuben J. Mapletoft, Ph.D. of the Western College of Veterinary Medicine, University of Saskatchewan, for contributing their time and expertise to the development of this page. Many synchronization programs have failed due to inadequate attention to detail. The period of time between removing the semen from the tank and depositing the semen in the cow should not exceed 15 minutes. Virginia Cooperative Extension Service. With pasture breeding, it is difficult for a bull to sire more than 40 calves in a 6 to 8 week calving period. Another criticism is that all calves will be born on the same day. Although artificial insemination (AI) of cattle has been possible for 60 years, this technology has not been used widely in the Canadian beef industry. The timing of insemination is critical to achieving high pregnancy rates.

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