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Nov 28

[Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec412 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11978 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec011106 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec093013 [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12394 The following transfer buffer recipes are provided to allow preparation of buffers from scratch. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec9042 [Full Text], Transfer buffer for submerged electroblotting of 20-400-kD proteins, Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10426 If you are transferring 2 gels, increase the methanol content to 20% to ensure efficient transfer. 2O • 100 mL 10x Transfer buffer • 200 mL methanol 20x TBS: For 4 L • 193.6 g Tris base • 640 g NaCl • Bring up the volume to 3.2 L with ddH 2O • Adjust the pH to 7.6 with concentrated HCl • Bring up the volume to 4 L with ddH [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11667 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec087874 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8645 Next lay filter paper, gel, and … [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12095 3. [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12277 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec273 [Full Text], Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec092320 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12023 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11969 [Full Text], Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.rec096024 Mix well and filter. Use a clean spatula for each ingredient or carefully pour each ingredient from its bottle. [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10711 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12290 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10513 [Full Text], Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec089284 Use the tabs below to see the recommended transfer buffers and transfer conditions for the gel chemistry being used for western blot wet or semi-dry transfer. [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12256 [Full Text], Transfer buffer for submerged electroblotting of proteins >80 kD, Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10427 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10629 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec696 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec9196 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11158 [Full Text], Transfer Buffer 1 (for Wet Transfer of Proteins 20–400 kDa), Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.rec100859 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec079574 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11161 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11118 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec088716 [Full Text], Tyrode Solution for Probing Ca2+ Signaling, Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec077859 [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec067264 Copyright © 2020 by Cold Spring Harbor Laboratory Press. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec418 [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12434 pH. 2) Add ddH 2 O to a final volume of 2 L. ** To make 1X Transfer Buffer from 10X: Mix 100 ml of 10X Transfer Buffer, 100 ml of methanol and 800 ml of ddH 2 … [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12056 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec629 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec083378 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10534 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12312 Search The pH listed for each buffer is for the 1X solution.

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